Well yes that's how multiplying by 10 works.
But measured levels in organs in the chart are on the order of 1/thousandth of that, all except levels in the injection site. E.g. 0.349 is 1000 times less than 349.
But measured levels in organs in the chart are on the order of 1/thousandth of that, all except levels in the injection site. E.g. 0.349 is 1000 times less than 349.
Then assume there is 0.349 mcg lipid in every g of tissue, how many lnp would be in 0.349 mcg? What is the weight of a single lnp in mcg? What is the weight of a cell in mcg?
Also judging by the table of the Japanese data about the LNP distribution in rats, are those numbers the lipid concentration inside the inner lining of the blood vessels and capillaries in each site? Or they concentrated outside of the blood vessels? I have read that our blood don't directly come in contact with the connective tissues of our body like muscles. fats, and glands right? Those cells outside of the blood vessels normally get nutrients and proteins from the small pores on the capillaries' wall? Are capillaries the only type of blood vessels that have pores as the holes that allow small particles to pass through? Artery and veins don't have those holes on them?
According to his pdf 10 months ago, based on his calculation based on the mRNA, the number of "molecules" inside the Pfizer shot is 1.35*e13(thirteen trillion five hundred billion), while the number of LNP inside the Moderna shot is 4.67*e13(forty-six trillion seven hundred billion). So by reading his pdf, are those number referring to the total molecules of the MRNA nucleotides/amino acids, or the total lipid nanoparticles in one injection? Can you please tell me what is your understanding? Thank you.
Here are his own writings in French:
Calcul du nombre de mol├йcules dтАЩARNm dans une dose de vaccin
1- Nous obtenons la s├йquence nucl├йotidique pr├йcise des ARNm ├а partir du
site de partage de donn├йes acad├йmiques github.com:
Thank you for the link! (and the links in the link).
If i understand correctly, the 30ug weight in one dose of Moderna is the combined weight of modRNA + lipids + nanoparticles etc. Then he divides by the mDaltons of the RNA itself, but not accounting for the weight of the lipids and LNPs etc, the proportion of which is unknown?
Not sure, my French is not very good.
As far as I think I know:
1) Moderna considers the exact formulation to be a proprietary trade secret.
2) Even if they gave us exact composition, molecular weights and percentages, why would we believe them?
3) Can we assume the production vials are the same as reference/testing vials?
4) McCairn and some other researcher did spectroscopy on vials and found no evidence for modRNA in them. (Maybe some are delivered as blanks?)
5) Even knowing the count of modRNA particles, we have no knowledge of how many cells get transfected, and whether this depends on an intramuscular injection or hitting a vein/artery.
I don't see any way for us plebes to approach a good estimate of number of cells transfected in-vivo.
Well, 30mcg is the dosage of the Pfizer mRNA, while Moderna has 50 mcg. Where did you read about the 30mcg being the total weight of the lipid nanoparticles and the gene sequences combined? I thought that's only the total mRNA materials' weight in the vial without the lipids?
I also read it online that the weight of a sperm cell is about 2X10^-14 kg, so looks like we can do some math here: 1 mcg = 1e-9 kg, therefore a sperm cell basically weights 2e-5 mcg, then 30mcg equals 1,500,000 sperms. While we don't know how much a lipid nanoparticle weight, we do know that a sperm cell is 30 times bigger than a lipid nanoparticle, so if the 30mcg is the total weight of all the LNP and mRNA combined in one dose, then there will be a total of 45,000,000 lipid nanoparticles in one injection, or forty five million per shot, which is way less than the number people online have been saying like trillions.
Well yes that's how multiplying by 10 works.
But measured levels in organs in the chart are on the order of 1/thousandth of that, all except levels in the injection site. E.g. 0.349 is 1000 times less than 349.
Then assume there is 0.349 mcg lipid in every g of tissue, how many lnp would be in 0.349 mcg? What is the weight of a single lnp in mcg? What is the weight of a cell in mcg?
Also interesting question. I don't know.
Here's the composition of pfizer/biontech and moderna LNP's. Both contain four different lipids.
https://www.cas.org/resource/blog/understanding-nanotechnology-covid-19-vaccines
https://www.sciencedirect.com/science/article/pii/S0378517321003914
Also judging by the table of the Japanese data about the LNP distribution in rats, are those numbers the lipid concentration inside the inner lining of the blood vessels and capillaries in each site? Or they concentrated outside of the blood vessels? I have read that our blood don't directly come in contact with the connective tissues of our body like muscles. fats, and glands right? Those cells outside of the blood vessels normally get nutrients and proteins from the small pores on the capillaries' wall? Are capillaries the only type of blood vessels that have pores as the holes that allow small particles to pass through? Artery and veins don't have those holes on them?
They used all 4 lipids in every single dose of their jabs? Do they all have the size around 60~100nm?
I dont know. I didn't make them. The size can be estimated from the chemical formula.
I see, thank you. So is my understanding about capillaries pores correct?
I don't know the dynamics of how adenovirus or LNP+modRNA permeates blood vessle walls.
Thank you for the insights. I have re-read this pdf written by Dr Claverie from France:
https://media-exp1.licdn.com/dms/document/C4E1FAQEdCOYYF1sDAw/feedshare-document-pdf-analyzed/0/1638640262719?e=1665014400&v=beta&t=7lBFvBp2mEG74Nqw0kz_fMdkkkQ_qO0nkXmh78YuIYQ
According to his pdf 10 months ago, based on his calculation based on the mRNA, the number of "molecules" inside the Pfizer shot is 1.35*e13(thirteen trillion five hundred billion), while the number of LNP inside the Moderna shot is 4.67*e13(forty-six trillion seven hundred billion). So by reading his pdf, are those number referring to the total molecules of the MRNA nucleotides/amino acids, or the total lipid nanoparticles in one injection? Can you please tell me what is your understanding? Thank you.
Here are his own writings in French:
Calcul du nombre de mol├йcules dтАЩARNm dans une dose de vaccin
1- Nous obtenons la s├йquence nucl├йotidique pr├йcise des ARNm ├а partir du
site de partage de donn├йes acad├йmiques github.com:
Assemblies-of-putative-SARS-CoV2-spike-encoding-mRNAsequences-for-vaccines-BNT-162b2-and-mRNA-1273
2- Nous calculons la masse molaire de chacun des ARNs (voir annexe):
BioNTech/Pfizer_BNT-162b2_vaccine: 1341,379 kDa (1.341.379 Da)
Moderna_mRNA-1273_vaccine: 1288,795 kDa (1.288.795 Da)
3- Nous calculons ├а combien de mol├йcules r├йelles correspondent les dosages indiqu├йs (30 microgrammes et 100 microgrammes)
Pfizer :
6.02 1023 (nombre dтАЩAvogadro) mol├йcules correspondent ├а 1.341.379 g
30 microgrammes correspondent donc ├а :
6.02 1023
1.341.379 ├Ч 30 ├Ч 10тИТ6 = 1.35 ├Ч 10^13
Moderna :
6.02 10^23 (nombre dтАЩAvogadro) mol├йcules correspondent ├а 1.341.379 g
100 microgrammes correspondent donc ├а :
6.02 10^23
1.288.795 ├Ч 100 ├Ч 10тИТ6 = 4.67 ├Ч 10^13
Comparaison avec le nombre de virus dans le cas dтАЩinfection naturelle
Les nombres ci-dessus sont v├йritablement astronomiques, quand on sait quтАЩun
patient est consid├йr├й comme tr├иs positif avec une valeur de PCR Ct=24, et que
cela correspond ├а 2.106virus/ml (millilitre)
Thank you for the link! (and the links in the link).
If i understand correctly, the 30ug weight in one dose of Moderna is the combined weight of modRNA + lipids + nanoparticles etc. Then he divides by the mDaltons of the RNA itself, but not accounting for the weight of the lipids and LNPs etc, the proportion of which is unknown?
Not sure, my French is not very good.
As far as I think I know:
1) Moderna considers the exact formulation to be a proprietary trade secret.
2) Even if they gave us exact composition, molecular weights and percentages, why would we believe them?
3) Can we assume the production vials are the same as reference/testing vials?
4) McCairn and some other researcher did spectroscopy on vials and found no evidence for modRNA in them. (Maybe some are delivered as blanks?)
5) Even knowing the count of modRNA particles, we have no knowledge of how many cells get transfected, and whether this depends on an intramuscular injection or hitting a vein/artery.
I don't see any way for us plebes to approach a good estimate of number of cells transfected in-vivo.
Well, 30mcg is the dosage of the Pfizer mRNA, while Moderna has 50 mcg. Where did you read about the 30mcg being the total weight of the lipid nanoparticles and the gene sequences combined? I thought that's only the total mRNA materials' weight in the vial without the lipids?
I also read it online that the weight of a sperm cell is about 2X10^-14 kg, so looks like we can do some math here: 1 mcg = 1e-9 kg, therefore a sperm cell basically weights 2e-5 mcg, then 30mcg equals 1,500,000 sperms. While we don't know how much a lipid nanoparticle weight, we do know that a sperm cell is 30 times bigger than a lipid nanoparticle, so if the 30mcg is the total weight of all the LNP and mRNA combined in one dose, then there will be a total of 45,000,000 lipid nanoparticles in one injection, or forty five million per shot, which is way less than the number people online have been saying like trillions.
Oh if the 30/50 mcg is the weight of the mRNA, then I misunderstood.
I thought it was the weight of all ingredients (minus water).