I don't know what relationship 'lipid equivalent' has to 'lipid'.
But yes you read it correctly. μg is 1 millionth of a gram. So most measured concentrations were around a few tenths of a millionth by weight or volume.
From the table, most concentrations seem to peak around 2 hours. Interesting exception is in the Eyes where it keep…
I don't know what relationship 'lipid equivalent' has to 'lipid'.
But yes you read it correctly. μg is 1 millionth of a gram. So most measured concentrations were around a few tenths of a millionth by weight or volume.
From the table, most concentrations seem to peak around 2 hours. Interesting exception is in the Eyes where it keeps growing to 48h. It seems oddly persistent in Lungs as well.
Come to think of it, i'm not sure I want to know how they measured concentrations of LNP in someone's eye >_<
But measured levels in organs in the chart are on the order of 1/thousandth of that, all except levels in the injection site. E.g. 0.349 is 1000 times less than 349.
Then assume there is 0.349 mcg lipid in every g of tissue, how many lnp would be in 0.349 mcg? What is the weight of a single lnp in mcg? What is the weight of a cell in mcg?
Also judging by the table of the Japanese data about the LNP distribution in rats, are those numbers the lipid concentration inside the inner lining of the blood vessels and capillaries in each site? Or they concentrated outside of the blood vessels? I have read that our blood don't directly come in contact with the connective tissues of our body like muscles. fats, and glands right? Those cells outside of the blood vessels normally get nutrients and proteins from the small pores on the capillaries' wall? Are capillaries the only type of blood vessels that have pores as the holes that allow small particles to pass through? Artery and veins don't have those holes on them?
According to his pdf 10 months ago, based on his calculation based on the mRNA, the number of "molecules" inside the Pfizer shot is 1.35*e13(thirteen trillion five hundred billion), while the number of LNP inside the Moderna shot is 4.67*e13(forty-six trillion seven hundred billion). So by reading his pdf, are those number referring to the total molecules of the MRNA nucleotides/amino acids, or the total lipid nanoparticles in one injection? Can you please tell me what is your understanding? Thank you.
Here are his own writings in French:
Calcul du nombre de molécules d’ARNm dans une dose de vaccin
1- Nous obtenons la séquence nucléotidique précise des ARNm à partir du
site de partage de données académiques github.com:
Thank you for the link! (and the links in the link).
If i understand correctly, the 30ug weight in one dose of Moderna is the combined weight of modRNA + lipids + nanoparticles etc. Then he divides by the mDaltons of the RNA itself, but not accounting for the weight of the lipids and LNPs etc, the proportion of which is unknown?
Not sure, my French is not very good.
As far as I think I know:
1) Moderna considers the exact formulation to be a proprietary trade secret.
2) Even if they gave us exact composition, molecular weights and percentages, why would we believe them?
3) Can we assume the production vials are the same as reference/testing vials?
4) McCairn and some other researcher did spectroscopy on vials and found no evidence for modRNA in them. (Maybe some are delivered as blanks?)
5) Even knowing the count of modRNA particles, we have no knowledge of how many cells get transfected, and whether this depends on an intramuscular injection or hitting a vein/artery.
I don't see any way for us plebes to approach a good estimate of number of cells transfected in-vivo.
I don't know what relationship 'lipid equivalent' has to 'lipid'.
But yes you read it correctly. μg is 1 millionth of a gram. So most measured concentrations were around a few tenths of a millionth by weight or volume.
From the table, most concentrations seem to peak around 2 hours. Interesting exception is in the Eyes where it keeps growing to 48h. It seems oddly persistent in Lungs as well.
Come to think of it, i'm not sure I want to know how they measured concentrations of LNP in someone's eye >_<
So assume there are 349 mcg in 1 g, if the organ weights 10 grams total, there will be 3940 mcg of lnp in it?
Well yes that's how multiplying by 10 works.
But measured levels in organs in the chart are on the order of 1/thousandth of that, all except levels in the injection site. E.g. 0.349 is 1000 times less than 349.
Then assume there is 0.349 mcg lipid in every g of tissue, how many lnp would be in 0.349 mcg? What is the weight of a single lnp in mcg? What is the weight of a cell in mcg?
Also interesting question. I don't know.
Here's the composition of pfizer/biontech and moderna LNP's. Both contain four different lipids.
https://www.cas.org/resource/blog/understanding-nanotechnology-covid-19-vaccines
https://www.sciencedirect.com/science/article/pii/S0378517321003914
Also judging by the table of the Japanese data about the LNP distribution in rats, are those numbers the lipid concentration inside the inner lining of the blood vessels and capillaries in each site? Or they concentrated outside of the blood vessels? I have read that our blood don't directly come in contact with the connective tissues of our body like muscles. fats, and glands right? Those cells outside of the blood vessels normally get nutrients and proteins from the small pores on the capillaries' wall? Are capillaries the only type of blood vessels that have pores as the holes that allow small particles to pass through? Artery and veins don't have those holes on them?
They used all 4 lipids in every single dose of their jabs? Do they all have the size around 60~100nm?
I dont know. I didn't make them. The size can be estimated from the chemical formula.
I see, thank you. So is my understanding about capillaries pores correct?
I don't know the dynamics of how adenovirus or LNP+modRNA permeates blood vessle walls.
Thank you for the insights. I have re-read this pdf written by Dr Claverie from France:
https://media-exp1.licdn.com/dms/document/C4E1FAQEdCOYYF1sDAw/feedshare-document-pdf-analyzed/0/1638640262719?e=1665014400&v=beta&t=7lBFvBp2mEG74Nqw0kz_fMdkkkQ_qO0nkXmh78YuIYQ
According to his pdf 10 months ago, based on his calculation based on the mRNA, the number of "molecules" inside the Pfizer shot is 1.35*e13(thirteen trillion five hundred billion), while the number of LNP inside the Moderna shot is 4.67*e13(forty-six trillion seven hundred billion). So by reading his pdf, are those number referring to the total molecules of the MRNA nucleotides/amino acids, or the total lipid nanoparticles in one injection? Can you please tell me what is your understanding? Thank you.
Here are his own writings in French:
Calcul du nombre de molécules d’ARNm dans une dose de vaccin
1- Nous obtenons la séquence nucléotidique précise des ARNm à partir du
site de partage de données académiques github.com:
Assemblies-of-putative-SARS-CoV2-spike-encoding-mRNAsequences-for-vaccines-BNT-162b2-and-mRNA-1273
2- Nous calculons la masse molaire de chacun des ARNs (voir annexe):
BioNTech/Pfizer_BNT-162b2_vaccine: 1341,379 kDa (1.341.379 Da)
Moderna_mRNA-1273_vaccine: 1288,795 kDa (1.288.795 Da)
3- Nous calculons à combien de molécules réelles correspondent les dosages indiqués (30 microgrammes et 100 microgrammes)
Pfizer :
6.02 1023 (nombre d’Avogadro) molécules correspondent à 1.341.379 g
30 microgrammes correspondent donc à :
6.02 1023
1.341.379 × 30 × 10−6 = 1.35 × 10^13
Moderna :
6.02 10^23 (nombre d’Avogadro) molécules correspondent à 1.341.379 g
100 microgrammes correspondent donc à :
6.02 10^23
1.288.795 × 100 × 10−6 = 4.67 × 10^13
Comparaison avec le nombre de virus dans le cas d’infection naturelle
Les nombres ci-dessus sont véritablement astronomiques, quand on sait qu’un
patient est considéré comme très positif avec une valeur de PCR Ct=24, et que
cela correspond à 2.106virus/ml (millilitre)
Thank you for the link! (and the links in the link).
If i understand correctly, the 30ug weight in one dose of Moderna is the combined weight of modRNA + lipids + nanoparticles etc. Then he divides by the mDaltons of the RNA itself, but not accounting for the weight of the lipids and LNPs etc, the proportion of which is unknown?
Not sure, my French is not very good.
As far as I think I know:
1) Moderna considers the exact formulation to be a proprietary trade secret.
2) Even if they gave us exact composition, molecular weights and percentages, why would we believe them?
3) Can we assume the production vials are the same as reference/testing vials?
4) McCairn and some other researcher did spectroscopy on vials and found no evidence for modRNA in them. (Maybe some are delivered as blanks?)
5) Even knowing the count of modRNA particles, we have no knowledge of how many cells get transfected, and whether this depends on an intramuscular injection or hitting a vein/artery.
I don't see any way for us plebes to approach a good estimate of number of cells transfected in-vivo.